Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring

Table 2

Antibody specifications–naïve/memory panel. Antibody-fluorochrome conjugates for naïve/memory T cell phenotype panel. The panel was composed of a backbone dried mixture of five antibodies (a+b) and the drop-in liquid markers: live/dead discriminator for PBMC (a) and anti-CD45 APC-Cy7 for WB (b) samples.

	Marker	Fluorochrome	Clone	Sample	Supplier	Format
a+b	CD4	FITC	13B8.2	PBMC/WB	Beckman Coulter	DURAClone custom backbone (dried)
	CCR7 (CD197)	PE	G043H7	PBMC/WB
	CD8	PeCy5.5	B9.11	PBMC/WB
	CD3	PeCy7	UCHT-1	PBMC/WB
	CD45RA	APC	2H4	PBMC/WB
a	Dead exclusion marker	Near-IR (NiR)	Live/dead; Fixable dead cell stain kit	PBMCs	Thermo Fisher	Drop-in (liquid)
b	CD45	APC-Cy7	2D1	WB	BioLegend	Drop-in (liquid)