A Multiple Antigen Peptide Vaccine Containing CD4⁺ T Cell Epitopes Enhances Humoral Immunity against Trichinella spiralis Infection in Mice

Figure 4

Cytokines secreted by splenocytes from immunized mice upon stimulation of mixed peptides T2 and T5 in vitro. Splenocytes secreted IFN-γ (a), IL-2 (b), IL-4 (c), IL-5 (d), and IL-6 (e) were detected by ELISA seven days after the final immunization. Splenocytes of each sample were stimulated simultaneously with ConA (5 μg/ml) as positive controls. All cytokines were greatly elevated upon stimulation with ConA, and the highest levels were observed as follows: IFN-γ—1280 pg/ml; IL-2—640 pg/ml; IL-4—60 pg/ml; IL-5—125 pg/ml; and IL-6—857 pg/ml (data not shown in the figure). The results are shown as the (one representative experiment out of three). ().

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