Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-Myc/RNMT Axis

Figure 3

Impacts of B7-H6 depletion in GSLCs and 293T. (a) Cell proliferation of GSLCs transfected with siR-NC, siR-708, or siR-993 was measured by CCK-8 triplicated on 0, 48, 72, and 96 h. (b) Cell signaling proteins Akt and ERK were analyzed by WB in GSLCs transfected with siR-NC, siR-708, or siR-993, and housekeeping gene GAPDH was blotted as control. (c) mRNA expression of c-Myc was evaluated by RT-PCR in U87, GSLCs, and GSLCs transfected with siR-NC, siR-708, or siR-993, respectively (). (d) Pearson correlation analysis was performed between the mRNA expression of B7-H6 and c-Myc base on CGGA mRNA-Seq database. (e) B7-H6 expression was analyzed by western blot in control and B7-H6 KO 293T cells. Arrows pointed out the specific band of B7-H6. (f) Cell proliferation assay was performed on control and B7-H6 KO 293T cells. 2H3 and 2H7 were two different clones of B7-H6 deficient 293T cells (). Data of (a), (c), and (f) were . , , and .

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