Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-Myc/RNMT Axis

Table 3

Guide RNA and detection PCR primer sequence for B7-H6 knockout with CRISPR-Cas9.

Guide RNA	Sequence
Control	5-GTATTACTGATATTGGTGGG-3
Cas1	5-GGGTGACCACCACCTCACAT-3
Cas2	5-GAGCCATTGTGTCTCCATGG-3
PCR primer	Sequence
Forward	5-AACCCCTCAACATCACGTCT-3
Reverse	5-TGTTGCTAACCCCAACACCAT-3