The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice

Figure 1

Treatment with poly(I:C) induces increased relative numbers of NK cells in the liver but decrease in the blood. Naïve C57BL/6 mice ( mice/group) were treated with 200 μg poly(I:C) in 200 μl and then sacrificed 4 hr post poly(I:C) injection. Single-cell suspensions were prepared of PBL and liver and stained with antibodies against NK1.1, CD3, and CD11b. Samples were analyzed by 3-color flow cytometry. The frequency of NK cells (NK1.1⁺CD3⁻) and NKT cells (NK1.1⁺CD3⁺) is shown in (a). The frequencies of CD11b⁺NK1.1⁻ and CD11b⁺NK1.1⁺ cells are shown in (b).

(a)

(b)