The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice

Figure 3

Kinetics of NK subsets after the treatment with poly(I:C) in the PBL and liver. Naïve C57BL/6 mice ( mice/group) were treated with 200 μg poly(I:C) in 200 μl and then sacrificed after 1, 4, 8, and 12 hours after injection. Single-cell suspensions were prepared of the PBL and liver and stained with antibodies against NK1.1, CD3, CD11b, and CD11c and analyzed by 3-color flow cytometry. The kinetics of NK1.1⁺CD11b⁺, NK1.1⁺CD11c⁺, NK1.1⁺CD11b^-, NK1.1^-CD11b⁺, CD3⁺NK1.1⁺, and CD11c⁺CD11b⁺ are shown in the (a) PBL and (b) liver. The data represents the at each time point of each group. , .

(a)

(b)