Oral Immunotherapy Using Probiotic Ice Cream Containing Recombinant Food-Grade Lactococcus lactis Which Inhibited Allergic Responses in a BALB/c Mouse Model

Figure 9

Cytokine levels IL-4 (a), IFN-γ (b), and TGF-β (c) in supernatant of spleen cell culture after the restimulation with rAma r 2. ns: not significant; , , and versus the normal or PBS-treated group. Normal group: untreated and unsensitized group (PBS and alum-sensitive and treated with PBS), PBS-treated group: sensitized with Ama r 2 and PBS-treated, rAma r 2 group: sensitized with Ama r 2 and treated with pure rAma r 2 at a concentration of 40 μg/ml, ice cream (20 μl): sensitized with rAma r 2 and treated with 20 μl ice cream containing r-L. lactis NZ1330, ice cream (50 μl): sensitized with rAma r 2 and treated with 50 μl of ice-cream containing probiotic bacteria, ice cream (100 μl): sensitized with rAma r 2 and treated with 100 μl of ice cream containing r-L. lactis NZ1330, r-L. lactis NZ1330 (Ama r 2 gene): sensitisize with Ama r 2 and treated with probiotic bacteria r-L. lactis NZ1330 which contains the Ama r 2, and r-L lactis NZ1330: sensitisized with Ama r 2 and treated with probiotic bacteria lacking the Ama r 2 gene.

(a)

(b)

(c)