CpG-ODN Induces a Dose-Dependent Enrichment of Immunological Niches in the Spleen and Lungs of Neonatal Chicks That Correlates with the Protective Immunity against Escherichia coli

Figure 5

Survival pattern and reduction of the relative risk of mortality following the E.coli challenge. (a) Survival of broiler chickens following E. coli challenge. Groups of broiler chicken embryos at day 18 of incubation were injected with either 5, 10, 25, and 50 μg of CpG-ODN or sterile saline by the in ovo route and then challenged with either or  cfu of E.coli three days later, at the day of hatch. Survival graph was plotted based on the event of a death in birds following challenge for each day postchallenge in each group. Survival was significantly higher in the birds that received 25 μg or 50 μg of CpG-ODN compared to the group of birds that received saline ( and 0.0430, respectively). (b) The reduction of the relative risk of mortality compared to the saline control group following E. coli challenge.

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