TRPV Subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) Gene and Protein Expression in Patients with Ulcerative Colitis

Table 1

Primers designs from Universal ProbeLibrary.

Gene	GeneBank	Oligonucleotides	Probe UPL
TRPV2	NM_016113.4	5 ggtgttggcctgactgga 3; 3cagcccctgctactgagaa 5	#3
TRPV3	NM_145068.2	5aatctcgggctggttggt 3; 3 ccaacacgaaggcttctacttc 5	#81
TRPV4	NM_021625.4 NM_147204.2 NM_001177428.1 NM_001177431.1 NM_001177433.1	5 ccaggtaggcctcgatcc 3; 3 gctccttccagctgctctac 5	#66
TRPV5	NM_019841.4	5 tggggtctgttccagaattt 3; 3 ctgtccttcctggagcttgt 5	#82
TRPV6	NM_018646.2	5 gaaggagaggagactcccaga 3; 3 agagccgagatgagcagaac 5	#84
GAPDH	NM_002046.3	5 gcccaatacgaccaaatcc 3; 3agccacatcgctcagacac 5	#60

Oligonucleotides were designed considering all the alternative splicing variations.