IL-17 Affects the Progression, Metastasis, and Recurrence of Laryngeal Cancer via the Inhibition of Apoptosis through Activation of the PI3K/AKT/FAS/FASL Pathways

Figure 6

The role of silenced IL-17 in apoptosis of LC cells. (a) Transcriptional expression of IL-17. The real-time PCR revealed that there was a 63% decrease of IL-17 mRNA in LC cells transfected with IL-17 siRNA compared with the control. GAPDH was used as an internal control. One representative experiment of the three independent experiments is demonstrated. compared with the control group. The expression of IL-17 in LC cells transfected with siRNA targeting IL-17 was detected with a Western blotting assay. The protein level of IL-17 was reduced to 73% by IL-17 siRNA. Each sample was tested in triplicate. One representative experiment of the three independent experiments is demonstrated. compared with the control group. (b) siRNA-IL-17 promoted Hep-2 cell apoptosis (53.4% compared to 11.05% in control cells), while siRNA-IL-17 promoted Tu212 cell apoptosis (39.6% compared to 11.67% in control cells). One representative experiment of the three independent experiments is demonstrated. versus the control. (c) siRNA-IL-17 promoted LC cell apoptosis. The TUNEL assay was conducted to detect the apoptotic cells in Hep-2 cells. Representative TUNEL staining results of different groups are shown in a . (d) The percentages of apoptotic cells in all groups were calculated. Data are presented as . in each group. Experiments were biologically repeated in triplicate. compared to the control group.

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