Wogonin Suppresses IL-10 Production in B Cells via STAT3 and ERK Signaling Pathway

Figure 2

Wogonin suppressed the production of IL-10 in LPS-induced B cells. The purified CD19⁺ cells from the spleen were stimulated with or without LPS in the presence or absence of Wogonin (0, 12.5, 25, 50, and 100 μM) for 6 h, 12 h, 24 h, and 48 h, and the frequencies of IL-10-producing B cells were analyzed. (a) Representative plots of IL-10-producing B cells with LPS induced in the presence or absence of Wogonin. (b–e) Quantification and MFI of IL-10-producing B cells at 6 h, 12 h, 24 h, and 48 h, respectively. (f) The supernatants from the different culture conditions were assayed by ELISA for the production of IL-10. Data are expressed as of three independent experiments and compared with one-way ANOVA. ; ; ; ; ns: no significance.

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