Wogonin Suppresses IL-10 Production in B Cells via STAT3 and ERK Signaling Pathway

Figure 6

Effect of Wogonin on the relative transcription factor levels of IL-10 in B cells. B cells were cultured with or without LPS in the presence of Wogonin (0, 12.5, 25, and 50 μM) for 6 h. Total RNA was extracted by Trizol and then reverse-transcribed. (a–d) The mRNA levels of Gata3, Batf, c-maf, Ahr, Egr2, Foxp3, JunB, and Hif-1α were measured by RT-PCR. The results are representative of three repeated experiments. ; ; ns: no significance.

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