Journal of Immunology Research / 2020 / Article / Tab 10 / Research Article
Analysis of the Heterogeneity of CD4+ CD25+ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice Table 10 Analysis of composition and characteristics of frequency for the top twenty sequences and the usage of TRBV and TRBJ gene, CDR3 AA of CD4+ CD25+ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 3.
Sample TRV gene TRJ gene CDR3 sequences (AA) Frequency (%) Total frequency (%) M3-B (top20) TCRBV19-01 TCRBJ01-03 CASSTSSGNTLYF 1.217 4.441 TCRBV31-01 TCRBJ02-03 CAWSPGLGGSAETLYF 0.908 TCRBV13-02 TCRBJ02-07 CASGSAGGISYEQYF 0.344 TCRBV13-01 TCRBJ02-07 CASSEPGGIYEQYF 0.282 TCRBV19-01 TCRBJ02-01 CASSIKLGGYAEQFF 0.186 TCRBV13-03 TCRBJ02-07 CASSEGLGGVKQYF 0.158 TCRBV19-01 TCRBJ02-01 CASSKGQGRYAEQFF 0.138 TCRBV02-01 TCRBJ01-03 CASRPGQTGNTLYF 0.117 TCRBV19-01 TCRBJ01-05 CASSLSGLWRAPLF 0.117 TCRBV13-01 TCRBJ02-07 CASSETGTEQYF 0.110 TCRBV13-02 TCRBJ02-01 CASGGGRNYAEQFF 0.103 TCRBV31-01 TCRBJ01-03 CAWSRQVNSGNTLYF 0.089 TCRBV01-01 TCRBJ02-07 CTCSAVGGAREQYF 0.089 TCRBV20-01 TCRBJ02-07 CGATRDSSYEQYF 0.089 TCRBV19-01 TCRBJ01-04 CASSIVGISNERLFF 0.089 TCRBV14-01 TCRBJ01-03 CASSFTGAGNTLYF 0.083 TCRBV29-01 TCRBJ02-07 CALGQGYEQYF 0.083 TCRBV04-01 TCRBJ02-07 CASSGGVEQYF 0.083 TCRBV29-01 TCRBJ02-07 CASSLSGQGGEQYF 0.083 TCRBV01-01 TCRBJ01-04 CTCSASVSNERLFF 0.076 M3-L (top20) TCRBV19-01 TCRBJ01-03 CASSTSSGNTLYF 1.629 5.370 TCRBV31-01 TCRBJ02-03 CAWSPGLGGSAETLYF 0.504 TCRBV13-03 TCRBJ02-07 CASSEGLGGVKQYF 0.407 TCRBV13-01 TCRBJ02-07 CASSEPGGIYEQYF 0.388 TCRBV13-01 TCRBJ02-07 CASSDATGGATYEQYF 0.233 TCRBV13-02 TCRBJ02-07 CASGSAGGISYEQYF 0.233 TCRBV04-01 TCRBJ02-07 CASSGGVEQYF 0.194 TCRBV19-01 TCRBJ02-01 CASSIKLGGYAEQFF 0.174 TCRBV13-02 TCRBJ01-03 CASGETTNSGNTLYF 0.174 TCRBV20-01 TCRBJ01-02 CGARDNANSDYTF 0.155 TCRBV13-02 TCRBJ02-01 CASGGGRNYAEQFF 0.136 TCRBV20-01 TCRBJ01-02 CGARDNANSDYTF 0.136 TCRBV02-01 TCRBJ02-07 CASSQDRSSYEQYF 0.136 TCRBV02-01 TCRBJ02-07 CASSHRDWGYEQYF 0.136 TCRBV05-01 TCRBJ01-05 CASSQDDQAPLF 0.136 TCRBV13-01 TCRBJ01-05 CASSGSNNQAPLF 0.136 TCRBV20-01 TCRBJ01-03 CGARDNSGNTLYF 0.116 TCRBV13-01 TCRBJ01-05 CASSGAGTENQAPLF 0.116 TCRBV02-01 TCRBJ01-03 CASSQDHIGNTLYF 0.116 TCRBV02-01 TCRBJ02-07 CASSQGDSSYEQYF 0.116 M3-S (top20) TCRBV13-01 TCRBJ02-07 CASSEPGGIYEQYF 0.137 1.572 TCRBV19-01 TCRBJ01-03 CASSTSSGNTLYF 0.128 TCRBV13-02 TCRBJ02-07 CASGSAGGISYEQYF 0.124 TCRBV19-01 TCRBJ02-01 CASSKGQGRYAEQFF 0.106 TCRBV13-03 TCRBJ02-07 CASSEGLGGVKQYF 0.097 TCRBV13-02 TCRBJ01-04 CASGDWNERLFF 0.088 TCRBV31-01 TCRBJ01-01 CAWSPPTANTEVFF 0.084 TCRBV14-01 TCRBJ02-07 CASSLTGGEVEQYF 0.084 TCRBV31-01 TCRBJ01-03 CAWSRQVNSGNTLYF 0.084 TCRBV02-01 TCRBJ01-04 CASSAGRPNERLFF 0.066 TCRBV13-02 TCRBJ01-03 CASGETTNSGNTLYF 0.066 TCRBV01-01 TCRBJ02-07 CTCSARGLSYEQYF 0.066 TCRBV19-01 TCRBJ01-05 CASSLSGLWRAPLF 0.062 TCRBV20-01 TCRBJ01-03 CGARDNSGNTLYF 0.057 TCRBV04-01 TCRBJ02-07 CASSYWGGSYEQYF 0.057 TCRBV13-02 TCRBJ02-07 CASGGTGVYEQYF 0.057 TCRBV01-01 TCRBJ02-07 CTCSAVGGAREQYF 0.057 TCRBV19-01 TCRBJ01-04 CASSIVGISNERLFF 0.053 TCRBV13-02 TCRBJ02-02 CASGEWGKNTGQLYF 0.049 TCRBV02-01 TCRBJ02-01 CASSQEEGNYAEQFF 0.049
