Analysis of the Heterogeneity of CD4⁺CD25⁺ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice

Table 5

Analysis of composition and characteristics of frequency for the top ten sequences and the usage of TRBV and TRBJ gene, CDR3 AA of CD4⁺CD25⁺ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 1.

Sample	TRV gene	TRJ gene	CDR3 sequences (AA)	Frequency (%)	Total frequency (%)
M1-B (top10)	TCRBV31-01	TCRBJ01-05	CAWRGTFHNNQAPLF	1.181	4.979
	TCRBV13-01	TCRBJ02-07	CASSERLGGYEQYF	1.099
	TCRBV02-01	TCRBJ01-05	CASSQGQGANQAPLF	0.741
	TCRBV29-01	TCRBJ01-02	CASSLGTGNSDYTF	0.483
	TCRBV31-01	TCRBJ02-07	CAWSPGLGGRDEQYF	0.479
	TCRBV20-01	TCRBJ02-07	CGARTGQGSYEQYF	0.239
	TCRBV31-01	TCRBJ02-02	CAWKATGNTGQLYF	0.224
	TCRBV19-01	TCRBJ02-05	CASRDREGNQDTQYF	0.210
	TCRBV26-01	TCRBJ01-06	LCQQSTGSYNSPLYF	0.198
	TCRBV19-01	TCRBJ01-03	CASSTYRGLSGNTLYF	0.125
M1-L (top10)	TCRBV13-01	TCRBJ02-07	CASSERLGGYEQYF	0.531	2.917
	TCRBV02-01	TCRBJ01-05	CASSQGQGANQAPLF	0.427
	TCRBV31-01	TCRBJ01-05	CAWRGTFHNNQAPLF	0.375
	TCRBV13-02	TCRBJ02-01	CASGGDWGGNYAEQFF	0.323
	TCRBV13-02	TCRBJ01-04	CASGDISNERLFF	0.323
	TCRBV13-02	TCRBJ01-05	CASGDTNNQAPLF	0.198
	TCRBV20-01	TCRBJ02-07	CGARTGQGSYEQYF	0.198
	TCRBV31-01	TCRBJ02-07	CAWSPGLGGRDEQYF	0.198
	TCRBV19-01	TCRBJ01-04	CASSLDRINERLFF	0.177
	TCRBV29-01	TCRBJ01-02	CASSLGTGNSDYTF	0.167
M1-S (top10)	TCRBV20-01	TCRBJ02-01	CGARPWGGAEQFF	0.309	1.161
	TCRBV31-01	TCRBJ01-05	CAWRGTFHNNQAPLF	0.159
	TCRBV19-01	TCRBJ01-04	CASSTRTSNERLFF	0.109
	TCRBV04-01	TCRBJ02-01	CASSFRDRGYAEQFF	0.100
	TCRBV17-01	TCRBJ01-03	CASSSRTGSGNTLYF	0.092
	TCRBV31-01	TCRBJ02-07	CAWSLRTGGSSYEQYF	0.084
	TCRBV13-03	TCRBJ01-05	CASRGTGNNQAPLF	0.084
	TCRBV13-03	TCRBJ02-07	CASSDAGWGEGQYF	0.075
	TCRBV13-02	TCRBJ02-01	CASGGDWGGNYAEQFF	0.075
	TCRBV17-01	TCRBJ02-07	CASSRGPGTGYEQYF	0.075