Analysis of the Heterogeneity of CD4⁺CD25⁺ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice

Table 6

Analysis of composition and characteristics of frequency for the top ten sequences and the usage of TBBV and TRBJ gene, CDR3 AA of CD4⁺CD25⁺ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 2.

Sample	TRV gene	TRJ gene	CDR3 sequences (AA)	Frequency (%)	Total frequency (%)
M2-B (top10)	TCRBV02-01	TCRBJ02-07	CASSPGHYEQYF	0.467	2.489
	TCRBV02-01	TCRBJ02-07	CASSQAGTGVYEQYF	0.339
	TCRBV14-01	TCRBJ02-07	CASRGGGYEQYF	0.252
	TCRBV13-01	TCRBJ01-02	CASRTANSDYTF	0.223
	TCRBV24-01	TCRBJ02-07	CASSLGLGDYEQYF	0.215
	TCRBV19-01	TCRBJ02-07	CASSSGTGAYEQYF	0.215
	TCRBV05-01	TCRBJ02-07	CASSQVDWGGSYEQYF	0.198
	TCRBV19-01	TCRBJ02-07	CASRPGLGGYEQYF	0.198
	TCRBV05-01	TCRBJ02-07	CASSQEGVSYEQYF	0.194
	TCRBV16-01	TCRBJ02-07	CASSLETGAYEQYF	0.186
M2-L (top10)	TCRBV16-01	TCRBJ02-05	CASSSWGGQDTQYF	0.825	4.069
	TCRBV02-01	TCRBJ02-07	CASSQAGTGVYEQYF	0.770
	TCRBV14-01	TCRBJ01-02	CASSQRTDANSDYTF	0.440
	TCRBV31-01	TCRBJ02-03	CAWSPGTGGGAETLYF	0.422
	TCRBV13-02	TCRBJ01-04	CASGDEGHSNERLFF	0.312
	TCRBV20-01	TCRBJ01-04	CGAGDRGPNERLFF	0.293
	TCRBV01-01	TCRBJ02-07	CTCSAGQSSYEQYF	0.293
	TCRBV19-01	TCRBJ02-07	CASSTGSSYEQYF	0.275
	TCRBV05-01	TCRBJ02-03	CASSQENWGSAETLYF	0.220
	TCRBV19-01	TCRBJ02-01	CASSSRTGGYAEQFF	0.220
M2-S (top10)	TCRBV02-01	TCRBJ02-07	CASSPGHYEQYF	0.202	1.205
	TCRBV02-01	TCRBJ02-02	CASSQGDRVTGQLYF	0.143
	TCRBV13-03	TCRBJ02-07	CASRGPGQGGEQYF	0.119
	TCRBV02-01	TCRBJ02-07	CASSQGLGGSEQYF	0.119
	TCRBV19-01	TCRBJ01-05	CASTPGRNNQAPLF	0.114
	TCRBV31-01	TCRBJ01-03	CAQGQHLNSGNTLYF	0.114
	TCRBV02-01	TCRBJ02-07	CASSQAGTGVYEQYF	0.104
	TCRBV05-01	TCRBJ02-07	CASSQDGGRTYEQYF	0.104
	TCRBV02-01	TCRBJ02-07	CASSQNPGQGAYEQYF	0.099
	TCRBV05-01	TCRBJ01-05	CASSQGNNQAPLF	0.089