Analysis of the Heterogeneity of CD4⁺CD25⁺ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice

Table 7

Analysis of composition and characteristics of frequency for the top ten sequences and the usage of TBBV and TRBJ gene, CDR3 AA of CD4⁺CD25⁺ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 3.

Sample	TRV gene	TRJ gene	CDR3 sequences (AA)	Frequency (%)	Total frequency (%)
M3-B (top10)	TCRBV19-01	TCRBJ01-03	CASSTSSGNTLYF	1.217	3.575
	TCRBV31-01	TCRBJ02-03	CAWSPGLGGSAETLYF	0.908
	TCRBV13-02	TCRBJ02-07	CASGSAGGISYEQYF	0.344
	TCRBV13-01	TCRBJ02-07	CASSEPGGIYEQYF	0.282
	TCRBV19-01	TCRBJ02-01	CASSIKLGGYAEQFF	0.186
	TCRBV13-03	TCRBJ02-07	CASSEGLGGVKQYF	0.158
	TCRBV19-01	TCRBJ02-01	CASSKGQGRYAEQFF	0.138
	TCRBV02-01	TCRBJ01-03	CASRPGQTGNTLYF	0.117
	TCRBV19-01	TCRBJ01-05	CASSLSGLWRAPLF	0.117
	TCRBV13-01	TCRBJ02-07	CASSETGTEQYF	0.110
M3-L (top10)	TCRBV19-01	TCRBJ01-03	CASSTSSGNTLYF	1.629	4.091
	TCRBV31-01	TCRBJ02-03	CAWSPGLGGSAETLYF	0.504
	TCRBV13-03	TCRBJ02-07	CASSEGLGGVKQYF	0.407
	TCRBV13-01	TCRBJ02-07	CASSEPGGIYEQYF	0.388
	TCRBV13-01	TCRBJ02-07	CASSDATGGATYEQYF	0.233
	TCRBV13-02	TCRBJ02-07	CASGSAGGISYEQYF	0.233
	TCRBV04-01	TCRBJ02-07	CASSGGVEQYF	0.194
	TCRBV19-01	TCRBJ02-01	CASSIKLGGYAEQFF	0.174
	TCRBV13-02	TCRBJ01-03	CASGETTNSGNTLYF	0.174
	TCRBV20-01	TCRBJ01-02	CGARDNANSDYTF	0.155
M3-S (top10)	TCRBV13-01	TCRBJ02-07	CASSEPGGIYEQYF	0.137	0.998
	TCRBV19-01	TCRBJ01-03	CASSTSSGNTLYF	0.128
	TCRBV13-02	TCRBJ02-07	CASGSAGGISYEQYF	0.124
	TCRBV19-01	TCRBJ02-01	CASSKGQGRYAEQFF	0.106
	TCRBV13-03	TCRBJ02-07	CASSEGLGGVKQYF	0.097
	TCRBV13-02	TCRBJ01-04	CASGDWNERLFF	0.088
	TCRBV31-01	TCRBJ01-01	CAWSPPTANTEVFF	0.084
	TCRBV14-01	TCRBJ02-07	CASSLTGGEVEQYF	0.084
	TCRBV31-01	TCRBJ01-03	CAWSRQVNSGNTLYF	0.084
	TCRBV02-01	TCRBJ01-04	CASSAGRPNERLFF	0.066