Journal of Immunology Research / 2020 / Article / Tab 8 / Research Article
Analysis of the Heterogeneity of CD4+ CD25+ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice Table 8 Analysis of composition and characteristics of frequency for the top twenty sequences and the usage of TRBV and TRBJ gene, CDR3 AA of CD4+ CD25+ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 1.
Sample TRV gene TRJ gene CDR3 sequences (AA) Frequency (%) Total frequency (%) M1-B (top20) TCRBV31-01 TCRBJ01-05 CAWRGTFHNNQAPLF 1.181 6.043 TCRBV13-01 TCRBJ02-07 CASSERLGGYEQYF 1.099 TCRBV02-01 TCRBJ01-05 CASSQGQGANQAPLF 0.741 TCRBV29-01 TCRBJ01-02 CASSLGTGNSDYTF 0.483 TCRBV31-01 TCRBJ02-07 CAWSPGLGGRDEQYF 0.479 TCRBV20-01 TCRBJ02-07 CGARTGQGSYEQYF 0.239 TCRBV31-01 TCRBJ02-02 CAWKATGNTGQLYF 0.224 TCRBV19-01 TCRBJ02-05 CASRDREGNQDTQYF 0.210 TCRBV26-01 TCRBJ01-06 LCQQSTGSYNSPLYF 0.198 TCRBV19-01 TCRBJ01-03 CASSTYRGLSGNTLYF 0.125 TCRBV31-01 TCRBJ02-07 CAWSLRTGGSSYEQYF 0.123 TCRBV05-01 TCRBJ01-04 CASSQEGGEGERLFF 0.116 TCRBV20-01 TCRBJ02-01 CGARPWGGAEQFF 0.111 TCRBV02-01 TCRBJ01-05 CASSQETDRGQAPLF 0.111 TCRBV19-01 TCRBJ02-04 CASSIWDDQNTLYF 0.111 TCRBV19-01 TCRBJ01-04 CASSLDRINERLFF 0.109 TCRBV17-01 TCRBJ01-04 CASSRRGQGISNERLFF 0.104 TCRBV02-01 TCRBJ01-03 CASSPMTGTGNTLYF 0.094 TCRBV13-02 TCRBJ01-03 CASGDARNSGNTLYF 0.094 TCRBV19-01 TCRBJ01-04 CASSTRTSNERLFF 0.092 M1-L (top20) TCRBV13-01 TCRBJ02-07 CASSERLGGYEQYF 0.531 4.209 TCRBV02-01 TCRBJ01-05 CASSQGQGANQAPLF 0.427 TCRBV31-01 TCRBJ01-05 CAWRGTFHNNQAPLF 0.375 TCRBV13-02 TCRBJ02-01 CASGGDWGGNYAEQFF 0.323 TCRBV13-02 TCRBJ01-04 CASGDISNERLFF 0.323 TCRBV13-02 TCRBJ01-05 CASGDTNNQAPLF 0.198 TCRBV20-01 TCRBJ02-07 CGARTGQGSYEQYF 0.198 TCRBV31-01 TCRBJ02-07 CAWSPGLGGRDEQYF 0.198 TCRBV19-01 TCRBJ01-04 CASSLDRINERLFF 0.177 TCRBV29-01 TCRBJ01-02 CASSLGTGNSDYTF 0.167 TCRBV31-01 TCRBJ02-07 CAWSLRTGGSSYEQYF 0.156 TCRBV14-01 TCRBJ01-05 CASSLRDRGQAPLF 0.135 TCRBV01-01 TCRBJ01-03 CTCSGTGGSGNTLYF 0.135 TCRBV13-02 TCRBJ02-07 CASGDVGLSSYEQYF 0.135 TCRBV31-01 TCRBJ02-01 CAWSLFGGNYAEQFF 0.125 TCRBV20-01 TCRBJ02-01 CGARPWGGAEQFF 0.125 TCRBV05-01 TCRBJ02-01 CASSQEGGWGNYAEQFF 0.125 TCRBV13-02 TCRBJ02-07 CASGDAGGAYEQYF 0.125 TCRBV17-01 TCRBJ02-07 CASSRTGGSYEQYF 0.115 TCRBV02-01 TCRBJ01-05 CASSQETDRGQAPLF 0.115 M1-S (top20) TCRBV20-01 TCRBJ02-01 CGARPWGGAEQFF 0.309 1.847 TCRBV31-01 TCRBJ01-05 CAWRGTFHNNQAPLF 0.159 TCRBV19-01 TCRBJ01-04 CASSTRTSNERLFF 0.109 TCRBV04-01 TCRBJ02-01 CASSFRDRGYAEQFF 0.100 TCRBV17-01 TCRBJ01-03 CASSSRTGSGNTLYF 0.092 TCRBV31-01 TCRBJ02-07 CAWSLRTGGSSYEQYF 0.084 TCRBV13-03 TCRBJ01-05 CASRGTGNNQAPLF 0.084 TCRBV13-03 TCRBJ02-07 CASSDAGWGEGQYF 0.075 TCRBV13-02 TCRBJ02-01 CASGGDWGGNYAEQFF 0.075 TCRBV17-01 TCRBJ02-07 CASSRGPGTGYEQYF 0.075 TCRBV14-01 TCRBJ02-07 CASSFGDKYEQYF 0.075 TCRBV29-01 TCRBJ02-01 CASSLSMVGQFF 0.075 TCRBV31-01 TCRBJ02-01 CAWSLFGGNYAEQFF 0.067 TCRBV02-01 TCRBJ01-03 CASSTGVGNTLYF 0.067 TCRBV19-01 TCRBJ01-03 CASSSGVGNTLYF 0.067 TCRBV02-01 TCRBJ02-07 CASSQDSSYEQYF 0.067 TCRBV19-01 TCRBJ01-04 CASSIRTGNERLFF 0.067 TCRBV14-01 TCRBJ02-01 CASRRSYAEQFF 0.067 TCRBV13-02 TCRBJ01-03 CASGDARNSGNTLYF 0.067 TCRBV13-02 TCRBJ01-05 CASGDQQAPLF 0.067
