Journal of Immunology Research / 2020 / Article / Tab 9 / Research Article
Analysis of the Heterogeneity of CD4+ CD25+ T Cell TCR β CDR3 Repertoires in Breast Tumor Tissues, Lung Metastatic Tissues, and Spleens from 4T1 Tumor-Bearing BALB/c Mice Table 9 Analysis of composition and characteristics of frequency for the top twenty sequences and the usage of TRBV and TRBJ gene, CDR3 AA of CD4+ CD25+ T cells TCR β CDR3 repertoires in breast tumor tissues, lung metastatic tissues, and spleens from 4T1 tumor-bearing BALB/c mouse 2.
Sample TRV gene TRJ gene CDR3 sequences (AA) Frequency (%) Total frequency (%) M2-B (top20) TCRBV02-01 TCRBJ02-07 CASSPGHYEQYF 0.467 3.899 TCRBV02-01 TCRBJ02-07 CASSQAGTGVYEQYF 0.339 TCRBV14-01 TCRBJ02-07 CASRGGGYEQYF 0.252 TCRBV13-01 TCRBJ01-02 CASRTANSDYTF 0.223 TCRBV24-01 TCRBJ02-07 CASSLGLGDYEQYF 0.215 TCRBV19-01 TCRBJ02-07 CASSSGTGAYEQYF 0.215 TCRBV05-01 TCRBJ02-07 CASSQVDWGGSYEQYF 0.198 TCRBV19-01 TCRBJ02-07 CASRPGLGGYEQYF 0.198 TCRBV05-01 TCRBJ02-07 CASSQEGVSYEQYF 0.194 TCRBV16-01 TCRBJ02-07 CASSLETGAYEQYF 0.186 TCRBV19-01 TCRBJ02-07 CASSMGGLGYEQYF 0.174 TCRBV05-01 TCRBJ02-07 CASSQGHYEQYF 0.157 TCRBV02-01 TCRBJ02-07 CASSQNPGQGAYEQYF 0.149 TCRBV20-01 TCRBJ01-05 CGAPGQGNQAPLF 0.145 TCRBV01-01 TCRBJ01-05 CTCSEQGTNQAPLF 0.145 TCRBV19-01 TCRBJ01-05 CASTPGRNNQAPLF 0.145 TCRBV02-01 TCRBJ02-07 CASSQPAGSYEQYF 0.128 TCRBV19-01 TCRBJ02-07 CASSIEGRGVYEQYF 0.124 TCRBV26-01 TCRBJ02-07 LCQQSSPVYEQYF 0.124 TCRBV05-01 TCRBJ02-01 CASSQDWGDTYAEQFF 0.120 M2-L (top20) TCRBV16-01 TCRBJ02-05 CASSSWGGQDTQYF 0.825 6.012 TCRBV02-01 TCRBJ02-07 CASSQAGTGVYEQYF 0.770 TCRBV14-01 TCRBJ01-02 CASSQRTDANSDYTF 0.440 TCRBV31-01 TCRBJ02-03 CAWSPGTGGGAETLYF 0.422 TCRBV13-02 TCRBJ01-04 CASGDEGHSNERLFF 0.312 TCRBV20-01 TCRBJ01-04 CGAGDRGPNERLFF 0.293 TCRBV01-01 TCRBJ02-07 CTCSAGQSSYEQYF 0.293 TCRBV19-01 TCRBJ02-07 CASSTGSSYEQYF 0.275 TCRBV05-01 TCRBJ02-03 CASSQENWGSAETLYF 0.220 TCRBV19-01 TCRBJ02-01 CASSSRTGGYAEQFF 0.220 TCRBV01-01 TCRBJ02-01 CTCSADPAHYAEQFF 0.220 TCRBV24-01 TCRBJ02-07 CASSLWGYEQYF 0.220 TCRBV12-01 TCRBJ01-02 CASSLPGTGDSDYTF 0.220 TCRBV13-03 TCRBJ01-03 CASSDAGNSGNTLYF 0.202 TCRBV05-01 TCRBJ02-07 CASSQDWGGASYEQYF 0.183 TCRBV04-01 TCRBJ02-07 CASSYSEYEQYF 0.183 TCRBV26-01 TCRBJ02-07 LCQQSSPVYEQYF 0.183 TCRBV05-01 TCRBJ02-07 CASSQDGGRTYEQYF 0.183 TCRBV19-01 TCRBJ02-03 CASSVGGNAETLYF 0.183 TCRBV24-01 TCRBJ01-03 CASSCSSGNTLYF 0.165 M2-S (top20) TCRBV02-01 TCRBJ02-07 CASSPGHYEQYF 0.202 2.005 TCRBV02-01 TCRBJ02-02 CASSQGDRVTGQLYF 0.143 TCRBV13-03 TCRBJ02-07 CASRGPGQGGEQYF 0.119 TCRBV02-01 TCRBJ02-07 CASSQGLGGSEQYF 0.119 TCRBV19-01 TCRBJ01-05 CASTPGRNNQAPLF 0.114 TCRBV31-01 TCRBJ01-03 CAQGQHLNSGNTLYF 0.114 TCRBV02-01 TCRBJ02-07 CASSQAGTGVYEQYF 0.104 TCRBV05-01 TCRBJ02-07 CASSQDGGRTYEQYF 0.104 TCRBV02-01 TCRBJ02-07 CASSQNPGQGAYEQYF 0.099 TCRBV05-01 TCRBJ01-05 CASSQGNNQAPLF 0.089 TCRBV13-01 TCRBJ01-03 CASSDAGQNSGNTLYF 0.089 TCRBV05-01 TCRBJ02-07 CASSQEMGAYEQYF 0.084 TCRBV26-01 TCRBJ02-07 LCQQSSPVYEQYF 0.084 TCRBV17-01 TCRBJ02-07 CASSRLGASYEQYF 0.084 TCRBV02-01 TCRBJ01-03 CASSQDRAGNTLYF 0.079 TCRBV01-01 TCRBJ02-01 CTCSADPAHYAEQFF 0.079 TCRBV19-01 TCRBJ02-07 CASSTGSSYEQYF 0.079 TCRBV02-01 TCRBJ02-02 CASSQGDTGQLYF 0.074 TCRBV19-01 TCRBJ01-05 CASRGANNQAPLF 0.074 TCRBV04-01 TCRBJ01-03 CASSLGQSGNTLYF 0.074
