The Sufficient Immunoregulatory Effect of Autologous Bone Marrow-Derived Mesenchymal Stem Cell Transplantation on Regulatory T Cells in Patients with Refractory Rheumatoid Arthritis

Table 1

Primer sequences designed for SYBR® Green real-time PCR.

Gene name	Accession number	Sequence
T-bet-F	NM_013351.1	5-ATTGCCGTGACTGCCTACCAGA-3
T-bet-R		5-GGAATTGACAGTTGGGTCCAGG-3
GATA3-F	NM_001002295.1	5-ACCACAACCACACTCTGGAGGA-3
GATA3-R		5-TCGGTTTCTGGTCTGGATGCCT-3
ROR-γt-F	NM_005060.4	5-CCCTGACAGAGATAGAGCACC-3
ROR-γt-R		5-TTCCCACATCTCCCACATGG-3
FOXP3-F	NM_014009.3	5-GGCACAATGTCTCCTCCAGAGA-3; 5-CAGATGAAGCCTTGGTCAGTGC-3
FOXP3-R
GAPDH-F	NM_001289746.1	5-CACTAGGCGCTCACTGTTCTC-3
GAPDH-R		5-CCAATACGACCAAATCCGTTGAC-3

Note: F: forward primer; R: reverse primer; GATA3: GATA binding protein 3; ROR-γt: retinoic acid-related orphan receptor gamma t; FOXP3: forkhead box P3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.