SIRT5 Contributes to Colorectal Cancer Growth by Regulating T Cell Activity

Figure 4

Sirt5 KO naïve T cell activation is promoted in vitro. (a) Scheme of experimental design. Mouse naïve T cells were purified from spleens of WT/KO mice and activated by anti-CD3/CD28. After culturing for 0 h, 24 h, 48 h, and 72 h, cells were collected and analyzed by flow cytometry. (b) Representative histograms of CD69 of naïve CD8⁺ T (0 h) and CD8⁺ T cells activated for 24 h, 48 h, and 72 h using flow cytometry. (c) Frequency of cells highly expressing CD69 among CD8⁺ T cells cultured for 0 h, 24 h, 48 h, and 72 h. (d) Representative histograms of CD25 of naïve CD8⁺ T (0 h) and CD8⁺ T cells activated for 24 h, 48 h, and 72 h using flow cytometry. (e) Frequency of cells highly expressing CD25 among CD8⁺ T cells cultured for 0 h, 24 h, 48 h, and 72 h. (f) Representative histograms of CD69 of naïve CD4⁺ T (0 h) and CD4⁺ T cells activated for 24 h, 48 h, and 72 h using flow cytometry. (g) Frequency of cells highly expressing CD69 among CD4⁺ T cells cultured for 0 h, 24 h, 48 h, and 72 h. (h) Representative histograms of CD25 of naïve CD4⁺ T (0 h) and CD4+ T cells activated for 24 h, 48 h, and 72 h using flow cytometry. (i) Frequency of cells highly expressing CD25 among CD4⁺ T cells cultured for 0 h, 24 h, 48 h, and 72 h. The data are expressed as the of four independent experiments. ; ; two-tailed unpaired -test.

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