SIRT5 Contributes to Colorectal Cancer Growth by Regulating T Cell Activity

Figure 5

IFN-γ production is promoted in Sirt5 KO activated T cells. (a, b) Representative flow cytometry (a) and percentage (b) of IFN-γ^highCD8⁺ T cells activated for 0 h, 24 h, 48 h, and 72 h. (c, d) Representative flow cytometry (c) and percentage (d) of IFN-γ^highCD4⁺ T cells activated for 0 h, 24 h, 48 h, and 72 h. (e, f) Representative flow cytometry (e) and percentage (f) of the proportions of Th1 cells (CD4⁺IFN-γ⁺) induced from naive T cells from WT/KO mice. (g, h) Representative flow cytometry (g) and percentage (h) of the proportions of CD4⁺ Treg cells (CD4⁺Foxp3⁺). (i, j) Representative flow cytometry (i) and percentage (j) of the proportions of CTLs (CD8⁺IFN-γ⁺). (k, l) Representative flow cytometry (k) and percentage (l) of the proportions of CD8⁺ Treg cells (CD8⁺Foxp3⁺). (m) Level of IFN-γ in the supernatant of WT and Sirt5 KO T cells activated for 48 h. (n) Cell viability of colorectal cancer cells (CT26.WT) cultured for 24 h in medium supplemented with murine recombinant IFN-γ. Data were from four or five independent experiments. The data are expressed as the . ; ; n.s.: not significant; two-tailed unpaired -test.

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