Research Article
Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine
Figure 1
Immature DCs efficiently phagocytized antigenic peptides that are covalently linked with HB100-108. (a) A schematic diagram illustrates the synthetic peptides used in this study. Three HLA-A0201-restricted peptides (survivin, Her2, and CEA) were covalently linked with HB100-108 via double arginine (RR) residues as a protease-sensitive linker. (b) Immature moDCs were pulsed with 40 μg/ml of FITC fluorochrome-conjugated peptides±HB100-108 for 1 hour at 37°C. After a triple wash with PBS, the uptake was quantified by FACS analysis. To quench the extracellular FITC signal, 50 μg/ml of trypan blue was added to the cell suspension prior to flow cytometry analysis. Unpulsed DCs were used as the negative control. (c) Immunofluorescence microscopy images showing the uptake of FITC-peptides+HB100-108 by immature moDCs.
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