Research Article
Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine
Figure 4
Peptides+HB100-108/pulsed DCs induced the degranulation of CD8+ T cells and enhanced allogeneic T cells to secrete inflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB100-108 (each with 40 μg/ml). Cells were washed and matured in the presence of a maturation cocktail for 24 hours. (a) Allogeneic T cells were stimulated with empty DCs or peptides±HB100-108/pulsed DCs at cell ratio of 5 : 1 in the presence of GolgiStop and anti-CD107a-APC mAb. After incubation for 12 hours at 37°C, cells were collected and stained with anti-CD8-PE mAb and analyzed by flow cytometry. (b) Empty DCs or peptides±HB100-108/pulsed DCs were cocultured with responder allogeneic T cells at a cell ratio of 1 : 5 for 18 hours. Supernatants were collected at the end of culture, and cytokine production was detected using a cytometric bead array (CBA) kit. Results represent the of three independent experiments.
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