miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT

Figure 4

miR-24-3p regulated EMT through KLF8. (a, b) Expression of miR-24-3p (a) and KLF8 (b) mRNAs in the cell as indicated was detected by qPCR. Transfection of miR-24-3p mimics in A549 cells with KLF8 overexpression increased miR-24-3p expression and also decreased KLF8 mRNA expression. (c) Expression of KLF8, Snail, and E-cadherin in A549 cells with overexpression of KLF8, miR-24-3p, or their corresponding genes. (d) A schematic diagram for roles of miR-24-3p in lung adenocarcinoma and interactions with KLF8 and EMT programs. miR-24-3p suppressed KLF8 transcription directly, and the reduced KLF8 levels resulted in a decrease in Snail expression and an increase in E-cadherin expression, which in turn promoted EMT program and ultimately lead to enhanced metastasis of lung adenocarcinoma. Data are . .

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