A 7-Amino Acid Peptide Mimic from Hepatitis C Virus Hypervariable Region 1 Inhibits Mouse Lung Th9 Cell Differentiation by Blocking CD81 Signaling during Allergic Lung Inflammation

Figure 3

Suppression of Th9 cell differentiation from memory CD4⁺ T cells by 7P. (a) To determine whether 7P could inhibit memory CD4⁺ T cells, 6–8-week-old mice were immunized with OVA and alum (2 μg OVA/mouse) twice on days 1 and 2. After 14 days, CD3⁺CD4⁺CD62L^-CD44^+high CD45RO⁺ T cells (memory CD4⁺ T cells) from the collected spleen were isolated and restimulated by OVA with 7P, sham-7P, or vehicle for 4–5 days. (b–d) RNA was isolated from cells, and Il9 Il10 lrf4 mRNA was quantified by qPCR. Data were expressed as (SEM; mice/group from 1 to 3 independent experiments). compared with sham-7P and vehicle-treated mice.

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