ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells
CGRP evokes increases in the expression of iNOS, RIG-I, and NF-κB in microglial cells in a time-dependent manner. (a) Western blot analysis of iNOS, RIG-I, and NF-κB expression in microglial cells with treatment of CGRP at 0, 1, 2, 4, and 6 h, respectively. (b) Western blotting analyses for the effect of HDAC2 inhibitor VPA on CGRP-evoked iNOS, RIG-I, and NF-κB protein expression in microglial cells with treatment of CGRP and pretreatment with VPA. Relative amounts of proteins were calculated by normalizing to GAPDH. All values are expressed as the ( independent cell culture preparations, Mann-Whitney tests or Kruskal-Wallis tests). versus controls; # versus CGRP only groups.
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