Research Article
Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity
Table 3
Fluorochrome-antibody combinations used for flow cytometry.
| | Human | Fluorochrome | Clone | Company | Dilution in 100 μL staining volume | Catalogue number and RRID |
| | CD62L | FITC | DREG-56 | BioLegend | 1 : 100 (1 μL) | Cat# 304803, RRID:AB_314463 | | CD16 | PE | 3G8 | BioLegend | 1 : 100 (1 μL) | Cat# 980102, RRID:AB_2616616 | | CD56 | PE/dazzle | HCD56 | BioLegend | 1 : 40 (2.5 μL) | Cat# 362543, RRID:AB_2565921 | | CD14 | PerCP/Cy5.5 | HCD14 | BioLegend | 1 : 50 (2 μL) | Cat# 325621, RRID:AB_893252 | | CD3 | PerCP/Cy5.5 | OKT3 | BioLegend | 1 : 50 (2 μL) | Cat# 344807, RRID:AB_10641704 | | CD11b | PE/Cy7 | ICRF44 | BioLegend | 1 : 40 (2.5 μL) | Cat# 301321, RRID:AB_830643 | | CD45 | APC | 2D1 | BioLegend | 1 : 100 (1 μL) | Cat# 368511, RRID:AB_2566371 | | CD3 | BrilliantViolet605 | OKT3 | BioLegend | 1 : 70 (1.4 μL) | Cat# 317331, RRID:AB_2561376 | | CD4 | BrilliantViolet711 | RPA-T4 | BioLegend | 1 : 70 (1.4 μL) | Cat# 300536, RRID:AB_2632791 | | HLADR | BrilliantViolet711 | L243 | BioLegend | 1 : 70 (1.4 μL) | Cat# 301045, RRID:AB_11219195 | | CD19 | BrilliantViolet785 | HIB19 | BioLegend | 1 : 70 (1.4 μL) | Cat# 302239, RRID:AB_11218596 | | CD8 | Brilliant Violet785 | RPA-T8 | BioLegend | 1 : 70 (1.4 μL) | Cat# 301045, RRID:AB_11219195 |
|
|
