Journal of Immunology Research / 2020 / Article / Tab 3

Research Article

Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity

Table 3

Fluorochrome-antibody combinations used for flow cytometry.

HumanFluorochromeCloneCompanyDilution in 100 μL staining volumeCatalogue number and RRID

CD62LFITCDREG-56BioLegend1 : 100 (1 μL)Cat# 304803, RRID:AB_314463
CD16PE3G8BioLegend1 : 100 (1 μL)Cat# 980102, RRID:AB_2616616
CD56PE/dazzleHCD56BioLegend1 : 40 (2.5 μL)Cat# 362543, RRID:AB_2565921
CD14PerCP/Cy5.5HCD14BioLegend1 : 50 (2 μL)Cat# 325621, RRID:AB_893252
CD3PerCP/Cy5.5OKT3BioLegend1 : 50 (2 μL)Cat# 344807, RRID:AB_10641704
CD11bPE/Cy7ICRF44BioLegend1 : 40 (2.5 μL)Cat# 301321, RRID:AB_830643
CD45APC2D1BioLegend1 : 100 (1 μL)Cat# 368511, RRID:AB_2566371
CD3BrilliantViolet605OKT3BioLegend1 : 70 (1.4 μL)Cat# 317331, RRID:AB_2561376
CD4BrilliantViolet711RPA-T4BioLegend1 : 70 (1.4 μL)Cat# 300536, RRID:AB_2632791
HLADRBrilliantViolet711L243BioLegend1 : 70 (1.4 μL)Cat# 301045, RRID:AB_11219195
CD19BrilliantViolet785HIB19BioLegend1 : 70 (1.4 μL)Cat# 302239, RRID:AB_11218596
CD8Brilliant Violet785RPA-T8BioLegend1 : 70 (1.4 μL)Cat# 301045, RRID:AB_11219195

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