Circulating Neutrophils of Nonalcoholic Steatohepatitis Patients Show an Activated Phenotype and Suppress T Lymphocytes Activity
Table 3
Fluorochrome-antibody combinations used for flow cytometry.
Human
Fluorochrome
Clone
Company
Dilution in 100 μL staining volume
Catalogue number and RRID
CD62L
FITC
DREG-56
BioLegend
1 : 100 (1 μL)
Cat# 304803, RRID:AB_314463
CD16
PE
3G8
BioLegend
1 : 100 (1 μL)
Cat# 980102, RRID:AB_2616616
CD56
PE/dazzle
HCD56
BioLegend
1 : 40 (2.5 μL)
Cat# 362543, RRID:AB_2565921
CD14
PerCP/Cy5.5
HCD14
BioLegend
1 : 50 (2 μL)
Cat# 325621, RRID:AB_893252
CD3
PerCP/Cy5.5
OKT3
BioLegend
1 : 50 (2 μL)
Cat# 344807, RRID:AB_10641704
CD11b
PE/Cy7
ICRF44
BioLegend
1 : 40 (2.5 μL)
Cat# 301321, RRID:AB_830643
CD45
APC
2D1
BioLegend
1 : 100 (1 μL)
Cat# 368511, RRID:AB_2566371
CD3
BrilliantViolet605
OKT3
BioLegend
1 : 70 (1.4 μL)
Cat# 317331, RRID:AB_2561376
CD4
BrilliantViolet711
RPA-T4
BioLegend
1 : 70 (1.4 μL)
Cat# 300536, RRID:AB_2632791
HLADR
BrilliantViolet711
L243
BioLegend
1 : 70 (1.4 μL)
Cat# 301045, RRID:AB_11219195
CD19
BrilliantViolet785
HIB19
BioLegend
1 : 70 (1.4 μL)
Cat# 302239, RRID:AB_11218596
CD8
Brilliant Violet785
RPA-T8
BioLegend
1 : 70 (1.4 μL)
Cat# 301045, RRID:AB_11219195
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