Research Article
Hypoxia Impairs NK Cell Cytotoxicity through SHP-1-Mediated Attenuation of STAT3 and ERK Signaling Pathways
Figure 2
Hypoxia decreases the expression level of NK cells cytotoxicity related molecules. (a) Flow cytometric analysis of granzyme B and perforin expression in KHYG-1 (upper panel) and NK92 (lower panel) cells, respectively. KHYG-1 and NK92 were cultured in normoxic (20% O2) and hypoxic (1% O2) for 24 h, then intracellular staining was performed to analyze the expression of granzyme and perforin quantitatively. Left panel: histogram overlays display representative examples of granzyme B and perforin expression analyzed in normoxic and hypoxic cell samples compared to the fluorescence minus one (FMO) control; Right panel: statistical analysis of the flow cytometry data (, , ). (b) Flow cytometric analysis of the intracellular level of IFN-γ in normoxic and hypoxic KHYG-1 and NK92 cells. Left panel: one of three representative flow cytometry results; Right panel: statistical analysis of the flow cytometry data (, , ). (c) Flow cytometric analysis of the membrane staining of degranulation marker CD107a in normoxic and hypoxic KHYG-1 and NK92 cells. Left panel: one of three representative flow cytometry results; Right panel: statistical analysis of the flow cytometry data (, , ). (d) Flow cytometric analysis of the membrane staining of activating receptor NKp30, NKp46, and NKG2D in normoxic and hypoxic KHYG-1 and NK92 cells. Left panel: one of three representative flow cytometry results; Right panel: statistical analysis of the flow cytometry data (, , ).
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