Hypoxia Impairs NK Cell Cytotoxicity through SHP-1-Mediated Attenuation of STAT3 and ERK Signaling Pathways

Figure 5

Inhibition of SHP-2 has no effect on NK cell cytotoxicity. (a) Western blotting shows the effects of SHP-2 inhibitor SHP099 on the phosphorylation of ERK and STAT3. Hypoxic KYHG-1 cells were pretreated with 5 μM SHP099 for 2 h, and then the cells were collected for Western blotting analysis. Representative Western blot images are shown in left panel; the densitometric analysis is shown in right panel (, , , ). (b) Flow cytometric analysis shows the effect of SHP099 on the cytotoxicity of KHYG-1 cells. KHYG-1 cells were pretreated with 5 μM SHP099 for 2 h, then incubated with K562 cells at different ratios for 4 h. (c) Statistical analysis of the effects of SHP099 on KHYG-1 cell cytotoxicity against K562 cells (, ns: no significance).

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