Comprehensive Screening of Mouse T-Cell Epitopes in Human Herpesvirus 6B Glycoprotein H/L/Q1/Q2 Tetramer Complex

Table 1

CD8⁺ T-cell short epitope peptide prediction in BALB/c mice.

Peptide no. (glycoprotein)	Length (aa)	Amino acid sequence^1	Estimated scores for restriction molecules^2			%rank
			BIMAS			NetMHC
			D^d	K^d	L^d	D^d	K^d	L^d
No. 13 (gH)	20	IVYSLNLYPSHGIYYIRVVE
	10	LYPSHGIYYI	10	2880	1.95	0.8	4	1.9
	9	LNLYPSHGI	0.5	80	1	27	10	37
	9	VYSLNLYPS	0.1	60	1	60	9.5	20
	9	LYPSHGIYY	2	60	3	0.7	17	13
	8	LYPSHGIY	2	60	—	3	18	35
	9	YPSHGIYYI	0.5	57.6	39	3.5	13	0.17
	8	NLYPSHGI	0.5	57.6	—	36	17	32
	10	SLNLYPSHGI	0.6	40	1	55	18	55
	9	HGIYYIRVV	6	20	2	7.5	33	50
	8	HGIYYIRV	6	20	—	12	55	37
No. 113 (gQ1)^3	20	RLKPLTAMTAIAFCPMTSKL
	9	AFCPMTSKL	1	1382.4	5	37	0.8	23
No. 157 (gQ2)	20	NGPRYLQMETFISDLFRYEC
	9	RYLQMETFI	0.3	5760	1	11	0.01	5
	8	RYLQMETF	0.18	144	—	9.5	0.8	16
	10	RYLQMETFIS	0.12	100	1	28	0.12	31
	10	LQMETFISDL	1.2	96	5	55	16	2.5
	9	TFISDLFRY	0.1	57.6	2	45	26	37
	9	QMETFISDL	1	48	1.5	60	12	4.5
	8	YLQMETFI	1.5	40	—	29	0.05	12
	10	TFISDLFRYE	0.01	6.912	0.1	55	33	75
	8	TFISDLFR	0.01	5.76	—	80	48	80
	8	NGPRYLQM	120	5	—	0.08	75	37
	10	GPRYLQMETF	0.36	1	90	1	75	8.5

¹ Underlined peptides were synthesised and used for experiments. Bold type peptides indicate determined short CD8⁺ T-cell epitope. ² No binding score. ³ AFCPMTSKL in No.113 was previously reported as the peptide including CD8⁺ T-cell epitope (Nagamata et al., 2019).