Linking Complement C3 and B Cells in Nasal Polyposis
Complement C3, C5, and anaphylatoxin receptor expression in CRSwNP and healthy tissue. (a) The lysed tissue of nasal polyps (NP), corresponding (cIT), and healthy inferior turbinates was analyzed for mRNA expression of complement proteins by qRT-PCR displaying mean 2-ΔCt values. Significant differences were detected for C5AR1 (NP vs. hIT ), C5L2 (NP vs. hIT /cIT vs. hIT ), C5 (NP vs. hIT /cIT vs. hIT ), C3AR (NP vs. hIT /NP vs. cIT ).. Means with SEM are shown. (b–f) Quantified protein expression of C3 (b, ), C3aR (c, ), C5 (d, ), C5aR1 (e), and C5L2 (f) comparing lysates of NP and cIT from CRSwNP patients () in Western blots under reducing conditions with four representative patient samples. Means with SEM are shown. ;;.
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