The change of phosphorylated proteins in tumor tissues after PZH treatment. (a) Scanned pictures of PEX100. The microarrays were scanned using a Sure Scan Dx Microarray Scanner, and the data quality of all sites was good. (b) The heat map was generated from normalized intensity data using the Morpheus tool (https://www.morpheusdata.com/
). The heat map represented fold change in phosphorylation status. Each cell in the heat map showed a ratio of phosphorylated to unphosphorylated proteins. Red indicated upregulation while green represented downregulation in phosphorylation of signaling proteins, and the intensity of color depended on the degree of phosphorylation. In the figure, we showed the heat map as three parts with the same groups to be more clear. (c) Protein regulation network was established by the Cytoscape tool (https://cytoscape.org/
) to demonstrate the interaction of phosphorylated proteins, and the central nodes were also shown visually in the networks. The color scheme was that red meant upregulation, green meant downregulation, and light blue meant no change.