B7-H4 Inhibits the Development of Primary Sjögren’s Syndrome by Regulating Treg Differentiation in NOD/Ltj Mice
B7-H4 suppresses the progression of pSS in NOD/Ltj mice. (a) B7-H4 expression in salivary glands from 8 weeks or 15 weeks of NOD/Ltj mouse was examined by IHC (100x). Arrows indicate B7-H4 expression. (b, c) Female NOD/Ltj mice were injected with IgG isotype ctrl or anti-B7-H4 mAb from 8 weeks of age. The mice were sacrificed at 15 weeks of age. H&E stains of the salivary gland sections from IgG isotype ctrl treatment and anti-B7-H4 mAb treatment mice. Arrows indicate infiltrates within the salivary gland (b). Histopathological assessment data are presented as FS (c). (d–f) Detection of pSS-associated autoantibodies in serum. Serum samples were collected from IgG isotype ctrl treatment and anti-B7-H4 mAb treatment NOD/Ltj mice, and the levels of immunoglobulins were measured by ELISA. Immunoglobulin levels for (d) IgM, (e) IgG, and (f) IgA are shown. Two-tailed Student’s -test. All the data presented were from three independent experiments.
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