B7-H4 Inhibits the Development of Primary Sjögren’s Syndrome by Regulating Treg Differentiation in NOD/Ltj Mice

Figure 3

B7-H4 regulates CD4+ T cell response in vivo. (a, b) Splenocytes from 15-week-old NOD/Ltj mice treated with IgG isotype ctrl and anti-B7-H4 mAb, respectively, were stained with anti-CD4/anti-intracellular Foxp3 antibodies and analyzed by flow cytometry (a), and accumulated data of CD4+Foxp3+/CD4+ T cells are shown (b). (c–e) Splenocytes from 15-week-old IgG isotype ctrl or anti-B7-H4 mAb treatment NOD/Ltj mice were stimulated with PMA and ionomycin for 4 h and were stained with anti-CD4/intracellular IFN-γ or anti-CD4/intracellular IL-17A antibodies and analyzed by flow cytometry (c), accumulated data of CD4+IFN-γ+/CD4+ T cells (d), and CD4+IL-17A+/CD4+ T cells (e). Two-tailed Student’s -test. All the data presented were from three independent experiments.

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