Interferon Kappa Is Important for Keratinocyte Host Defense against Herpes Simplex Virus-1

Figure 2

Silencing IFNκ leads to enhanced HSV-1 replication in NHEK. Undifferentiated (UD) and differentiated (D) NHEK cells were transfected with scrambled siRNA and IFNκ siRNA duplexes. The cells were then infected with HSV-1 for an additional 24 hours before harvested. (a) IFNκ transcripts and (b) HSV-1 gD transcripts were evaluated by real-time qRT-PCR. (c) Representative viral plaque assay results were shown. (d) Quantitative results of HSV-1 plaque assay using infectious materials collected from NHEK infected with HSV-1 (MOI of 0.05). (e) IFNκ transcripts and (f) HSV-1 gD transcripts were evaluated by real-time qRT-PCR. (g) Representative viral plaque assay results were shown. (h) Quantitative results of HSV-1 plaque assay using infectious materials collected from NHEK HSV-1 (MOI of 0.05). Data is presented as . One of four independent experiments is presented. ; .

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