Negative Effects of SIGIRR on TRAF6 Ubiquitination in Acute Lung Injury In Vitro

Figure 3

Effect of TRAF6 reversal of SIGIRR in AMCs. (a, b) SIGIRR overexpression was performed to detect changes on SIGIRR and TRAF6 in AECs and AMCs. (c, d) The mRNA levels of downstream NF-κB signaling molecules, including IL-6, IL-8, TNF-α, NFKBIA, NFKB1, and RelA, were observed in the presence or absence of SIGIRR_shRNA in AECs and AMCs. (e) The rescue experiments were performed by cotransfection with TRAF6_cDNA and SIGIRR_shRNA in AMCs. The data represent three independent experiments with four samples for each treatment. and .

(a)

(b)

(c)

(d)

(e)