β-Defensin Strengthens Antimicrobial Peritoneal Mast Cell Response

Figure 1

Constitutive and BD-2-induced TLR3, TLR7, TLR9, and RIG-I mRNA and protein expressions in PMCs. PMCs were incubated with medium alone (resting nonstimulated cells) or BD-2 at a final concentration of 1 μg/mL, and TLR3, TLR7, TLR9, and RIG-I expressions were assessed by (a) qRT-PCR and (b–f) flow cytometry. Representative flow cytometry histogram showing (b) TLR3, (c) TLR7, (d) TLR9, and (e) RIG-I expressions. Shaded tracings—isotype control and open tracings—receptor expression in resting cells (green), and after BD-2 stimulation for 1 h (violet) and 3 h (blue). (f) The effect of BD-2 on TLR3/7/9/RIG-I expression on rat MCs. Constitutive receptor expression served as a control and was referred to as 100%. Results are the of three experiments performed in duplicate. Differences were considered significant at and are labeled with an asterisk () on each graph (Student’s -test). , .

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