Innate Immune Effectors Play Essential Roles in Acute Respiratory Infection Caused by Klebsiella pneumoniae

Figure 4

Susceptibility of K. pneumoniae to complement-mediated killing. 50 μl of the bacteria suspension ( CFUs) was mixed with 50 μl of either active or heat-inactivated rabbit serum in 96-well microplates and incubated at 37°C for 1 h, and viable bacteria were quantified after culture overnight. Complement mediated killing was calculated as follows: , CFU_HI represents heat-inactivated number, and CFU_A indicates a complement-active number. This experiment was performed in triplicate, and representative data from one experiment were expressed as . Statistical analyses were performed by Student’s test. () compared to each other.