Intranigral Administration of β-Sitosterol-β-D-Glucoside Elicits Neurotoxic A1 Astrocyte Reactivity and Chronic Neuroinflammation in the Rat Substantia Nigra

Figure 2

Time course of OX42 immunoreactivity after a BSSG injection in the SNpc. (a) Representative micrographs of the double immunofluorescence for TH and OX42 in untreated rats (Ut) and rats at different times (shown at the left side of micrographs) after BSSG injection. Immunofluorescence area density (IFAD) for TH (b) and OX42 (c) was determined using the ImageJ software v.1.46r (National Institutes of Health, Bethesda, MD). The TH and OX42 values for the mock rats correspond to the quantification in Supplementary Figure 2. The values are the from four anatomical levels. independent rats in each time of each experimental condition. Two-way ANOVA and post hoc Bonferroni tests were applied for statistical analysis. () indicates a compared with the DMSO mock and Ut groups of the respective immunostaining.

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