Regulation of Innate Lymphoid Cells in Acute Kidney Injury: Crosstalk between Cannabidiol and GILZ

Figure 1

Effects of cannabidiol on ILCs subsets in the murine model of acute kidney injury. Mice were subjected to left renal ischemia- (20 min) reperfusion (1 day) injury (IRI) while the right kidney served as sham control; the animals were subdivided to receive intraperitoneal administration of either vehicle (DMSO) or cannabidiol (CBD), as described under Methods, before restoration of renal blood flow. Thereafter, cell preparations of kidneys from each animal were subjected to flow cytometry analyses for determination of ILC subsets using specific markers. (a) Gating strategy based on side scatter (SSC) vs. forward scatter (FSC) of kidney cells from experimental groups followed by identification of each subset of ILCs relying on phenotypic markers as described in Methods. (b–d) Quantitative data for each ILC subtype for each experimental group; data are individual values as well as of animals/group/condition; each independent experiment included 3-5 animals. compared to other three groups. ^# compared to their vehicle-treated counterparts. ^† compared to sham-operated groups.