Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells

<div>Differentiation of Th17 and Treg after exposure to gastrospheres and parental-derived condition media in the presence of anti-CD3/CD28 microbeads. (a) CD4<sup>+</sup>CD25<sup>+</sup>FoxP3<sup>+</sup> Treg cell differentiation after exposure to gastrospheres or parental cell-derived condition media. (b) CD4<sup>+</sup>IL-17<sup>+</sup> Th17 cell differentiation after exposure to gastrospheres or parental cell-derived conditioned media. (c) Th17 to Treg ratio after exposure to gastrospheres or parental cell-derived conditioned media. (d) Concentrations of IL-17, TGF-<i>β</i>, and IL-10 cytokines measured by ELISA in supernatants of PBMCs after being exposed to gastrospheres or parental cells’ conditioned media. CSC: gastrospheres. 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Journal of Immunology Research

fig3

Figure 3

Figure 3: Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells 