Research Article
Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells
Table 1
Primer sequences used for quantitative real-time polymerase chain reaction.
| | Primer name | Primer sequence |
| | IL-17 | F: 5 AACCGATCCACCTCACCTTG 3 | | R: 5 CCCACGGACACCAGTATCTT 3 | | IL-6 | F: 5 A G G A G A C T T G C C T G G T G A AA 3 | | R: 5 C A G GGG T G G T T A T T G C A T C T 3 | | IL-8 | F: 5 T A G C A AAA T T G A G G C C A A G G 3 | | R: 5 A G C A G A C T A G GG T T G C C A G A 3 | | IL-10 | F: AAG CTG AGA ACC AAG ACC CA | | R: AAG GCA TTC TTC ACC TGC TC | | IL-22 | F: TGTGAGCTCTTTCCTTATGG | | R: TGCGGTTGGTGATATAGGGC | | IL-23 | F: GCAGATTCCAAGCCTCAGTC | | R: CCTTGAGCTGCTGCCTTTAG | | STAT3 | F: 5 GAAGAATCCAACAACGGCAG 3 | | R: 5 TCACAATCAGGGAAGCATCAC 3 | | TNF-α | F: 5 CCTCTCTCTAATCAGCCCTCTG 3 | | R: 5 GAGGACCTGGGAGTAGATGAG 3 | | IFN-γ | F: 5 GGTTCTCTTGGCTGTTACTG 3 | | R: 5 TCTTTTGGATGCTCTGGTCA 3 | | TGF-β | F: 5 AACCCACAACGAAATCTATGAC 3 | | R: 5 TAACTTGAGCCTCAGCAGAC 3 |
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IL-17: interleukin 17; IL-8: interleukin 8; IL-6: interleukin 6; IL-10: interleukin 10; IL-22: interleukin 22; IL-23: interleukin 23; STAT3: signal transducer and activator of transcription 3; TNF-α: tumor necrosis factor alpha; IFN-γ: interferon gamma; TGF-β: transforming growth factor beta.
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