Research Article
Chloroquine and Rapamycin Augment Interleukin-37 Expression via the LC3, ERK, and AP-1 Axis in the Presence of Lipopolysaccharides
Figure 1
Effects of LPS and autophagy-modifying drugs on IL-37 and IL-1β expression in U937 cells. (a) Relative fold changes of IL-37 and IL-1β mRNA in U937 macrophages 2 h after treatments. Each sample was tested in triplicate, and the change folds were shown in (b) IL-37 and IL-1β intracellular staining in U937 macrophages at 24 h after treatments. Brefeldin A was used to block protein transport. The experiment was repeated twice and yielded similar results. The representative graphs of flow cytometry analysis are shown accompanied with IL37-positive and IL-1β-positive proportions in columns.
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