Chloroquine and Rapamycin Augment Interleukin-37 Expression via the LC3, ERK, and AP-1 Axis in the Presence of Lipopolysaccharides

Figure 4

IL-37 expression induced by LPS and autophagy-modifying reagents correlates with LC3 conversion. (a) The representative western blot images and (b) columns of relative densitometric data showing the content of Beclin1, p62, and LC3 in treated U937 cells. The experiment was repeated twice independently and yielded similar results. (c) Spearman’s analysis revealed a correlation between IL-37 mRNA and the LC3 II/I ratio only in the presence of LPS stimulation. (d) The representative images of LC3 immunofluorescence staining observed with a confocal microscope. (e) Comparison of IL-37 expression and IL-1β expression between Cyto-ID-positive cells and Cyto-ID-negative cells isolated by flow cytometry sorting (quantitation was repeated three times and presented as ). The ratio of IL-37 and IL-1β expression in Cyto-ID-positive cells to negative cells was also computed and plotted in the right.

(a)

(b)

(c)

(d)

(e)