A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice

Figure 7

HSD increases gp91phox-mediated ROS production following downregulation of CD122 in NK cells. Representative flow cytometry profiles and mean fluorescence intensity of T-bet (a), Eomes (b), E4BP4 (c), ROS (d), and CD122 (e). Expression levels of the NADPH oxidase subunits, gp91phox and p47phox, were detected by western blotting (f). Representative flow cytometry profiles and mean fluorescence intensity of ROS in NK cells following inhibition by N-acetylcysteine or induction by H₂O₂ (g). Representative flow cytometry profiles and mean fluorescence intensity of CD122 in NK cells following inhibition or induction of ROS (h). All the data represent at least three independent experiments. Data are shown as the . Unpaired Student’s -tests (two-tailed) were performed using the Prism software. A value of <0.05 was considered significant. , .

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