HMGB1 Recruits TET2/AID/TDG to Induce DNA Demethylation in STAT3 Promoter in CD4+ T Cells from aGVHD Patients
Interference with TET2/AID/TDG in aGVHD CD4+ T cells could induce DNA hypermethylation of STAT3 promoter. (a) Representative Western blot results for TET2, AID, TDG, and GAPDH levels. (b), (c) Quantitative analysis of the (b) relative protein levels and (c) relative mRNA levels of TET2, AID, and TDG in aGVHD CD4+ T cells transfected with TET2/AID/TDG interference plasmids or control plasmid. (d), (e) The binding levels of (d) TET2/AID/TDG in STAT3 promoter and (e) DNA methylation level of STAT3 promoter in TET2/AID/TDG-deficient aGVHD CD4+ T cells or control aGVHD CD4+ T cells. (f), (g) Quantitative analysis of the (f) relative protein levels and (g) relative mRNA levels of TET2, AID, and TDG in non-aGVHD CD4+ T cells transfected with TET2/AID/TDG overexpression plasmids or control plasmids. (h), (i) (h) The binding levels and (i) DNA methylation level in TET2/AID/TDG overexpressed non-aGVHD CD4+ T cells or control cells. Data represent the mean of three independent experiments (; ); , , .