Anti-Inflammatory Activity of Diterpenoids from Celastrus orbiculatus in Lipopolysaccharide-Stimulated RAW264.7 Cells

Figure 4

Compounds 1 and 3 suppressed MAPK signaling pathway. (a, e) Immunoblot analysis showed that phosphorylated protein levels of MAPK signaling cascades, JNK, ERK1/2, and p38 are inhibited by compounds 1 (a) and 3 (e) in RAW264.7 macrophages. (b–d, f–h) Total-JNK, ERK1/2, and p38 MAPK proteins were used as loading controls. (b, f) Cells were preincubated for 2 h with each compound 1 and 3 at concentrations of 5 and 10 μM, respectively, and stimulated with LPS (1 μg/mL) for 1 h. Dexamethasone served as the positive control. Immunoblot analysis performed triplicate experiments, and data represented . Significant difference was considered at the levels of ^##, ^###, , , and versus LPS.

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