Anti-Inflammatory Activity of Diterpenoids from Celastrus orbiculatus in Lipopolysaccharide-Stimulated RAW264.7 Cells

Figure 5

Compounds 1 and 3 attenuated the NF-κB signaling pathway. (a, d) Immunoblot analysis displayed that activation of the NF-κB signaling pathway was suppressed by compounds 1 (a) and 3 (d) in RAW264.7 cells. (b, c, e, f) The graph was expressed as the values of the relative ratio IκBα or p65 to β-actin protein expression level using densitometry. Cells were pretreated for 2 h with compounds 1 and 3 at concentrations of 5 and 10 μM, respectively, and stimulated with LPS (1 μg/mL) for 1 h. Dexamethasone was used as the positive control, and immunoblots analysis performed triplicate experiments. Values are , and an unpaired Student -test was used for statistical analysis. ^#, ^##, ^###, , , and represented significant differences from the LPS-treated group.

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