Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization

Figure 1

B4 decreased proinflammatory response in macrophages. (a) Chemical structure of B4. (b, c) J774A.1 and THP-1 cells were treated with B4 (5, 10, and 20 μM) for 24 h, and the cell viability was detected by MTT assay. (d) THP-1 cells were pretreated with B4 (5, 10, and 20 μM) for 4 h and subsequently cocultured with LPS (1 μg/mL) for an additional 18 h. Western blotting assay was used to detect the expression of iNOS and COX-2. (e–g) THP-1 cells treated with B4 for 4 h and cocultured with LPS (1 μg/mL) for 5 h, then stimulated with ATP (5 mM) for 30 min. The medium was collected, and the levels of TNF-α, IL-6, and IL-1β were detected by ELISA kit. All the experiments were performed three times independently. , , and compared with the model group.

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