Anemoside B4 Protects against Acute Lung Injury by Attenuating Inflammation through Blocking NLRP3 Inflammasome Activation and TLR4 Dimerization

Figure 2

B4 suppressed NLRP3 inflammasome activation. (a, b) J774A.1 cells and THP-1 cells were pretreated with B4 for 4 h, then cocultured with LPS (1 μg/mL) for another 5 h, and stimulated with ATP (5 mM) for 30 min. The expression levels of NLRP3, caspase-1, and IL-1β were determined by Western blotting. All the experiments were performed three times independently. and versus LPS+ATP group.

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